TRANSFER OF SMALL YACS TO ESCHERICHIA-COLI AS LARGE CIRCULAR PLASMIDS

Authors
FRENGEN E WU CY DEJONG PJ
Citation
E. Frengen et al., TRANSFER OF SMALL YACS TO ESCHERICHIA-COLI AS LARGE CIRCULAR PLASMIDS, GENET A-BIO, 14(2), 1997, pp. 55-59
Citations number
18
Categorie Soggetti
Genetics & Heredity","Biochemical Research Methods
Journal title
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING → ACNP
ISSN journal
10503862
Volume
14
Issue
2
Year of publication
1997
Pages
55 - 59
Database
ISI
SICI code
1050-3862(1997)14:2<55:TOSYTE>2.0.ZU;2-Q
Abstract
We have designed a YAC circularization vector, pCIRC3, allowing enrich ment of the YAC DNA by exonuclease digestion of the linear yeast chrom osomes. Due to the presence of P1 replicon sequences in this vector, t he circular YACs would replicate as PACs in Escherischia coli. (C) 199 7 Elsevier Science B.V.