A UNIVERSAL POSTEMBEDDING PROTOCOL FOR IMMUNOGOLD LABELING OF OSMIUM FIXED, EPOXY RESIN-EMBEDDED TISSUE

Authors
MORRIS RE CIRAOLO GM
Citation
Re. Morris et Gm. Ciraolo, A UNIVERSAL POSTEMBEDDING PROTOCOL FOR IMMUNOGOLD LABELING OF OSMIUM FIXED, EPOXY RESIN-EMBEDDED TISSUE, Journal of Electron Microscopy, 46(4), 1997, pp. 315-319
Citations number
8
Categorie Soggetti
Microscopy
Journal title
Journal of Electron Microscopy → ACNP
ISSN journal
00220744
Volume
46
Issue
4
Year of publication
1997
Pages
315 - 319
Database
ISI
SICI code
0022-0744(1997)46:4<315:AUPPFI>2.0.ZU;2-7
Abstract
The authors have found that double etching of epoxy-embedded, ultrathi n sections with 3% sodium metaperiodate rendered epitope expression co mparable to that obtained with either saturated or half-saturated sodi um metaperiodate solutions. In contrast to either of the two more conc entrated oxidizing solutions, double etching with 3% sodium metaperiod ate neither bleached the specimens nor generated holes in the plastic resin.