RIO-MAMORE VIRUS - GENETIC-CHARACTERIZATION OF A NEWLY RECOGNIZED HANTAVIRUS OF THE PYGMY RICE RAT, OLIGORYZOMYS-MICROTIS, FROM BOLIVIA

Human hantavirus disease occurs throughout much of South America. The rodent hosts and the specific etiologic agent(s) are largely unknown, but many reported cases occurred within the habitation ranges of oryzo mine rodents (rice rats). We have identified a genetically novel hanta virus (Rio Mamore virus [RM]) of the pygmy rice mt Oligoryzomys microt is in Bolivia. The complete sequence of the small (S) genome and the p artial sequence of the medium (M) genome are described. This virus is closely related to the newly identified human pathogen Andes virus fro m Patagonia. To facilitate improved diagnosis of hantavirus infections in South America, we have expressed the complete nucleocapsid protein of RM in Escherichia coli and affinity-purified it for use in an ELIS A and Western blot assays for antibodies to RM.