SIMULTANEOUS DETERMINATION OF FECAL FAT, NITROGEN, AND WATER BY NEAR-INFRARED REFLECTANCE SPECTROSCOPY

Background: Determinations of fecal fat and nitrogen reveal evidence o f malabsorption and assist in estimating the efficacy of pancreatic en zyme treatment. Seventy-two-hour stool collection. with chemical analy sis of fecal fat, and Kjeldahl's method for measurement of fecal nitro gen are generally accepted as standard methods for making these determ inations. However, these traditional methods are expensive, time-consu ming, and cumbersome. This study evaluated the efficiency and validity of an alternative method, using near-infrared reflectance spectroscop y (NIRS) and compared results with those of the standard methods. Meth ods: Near-infrared reflectance spectroscopy is a secondary method: The instrument first has to be calibrated with samples analyzed by the st andard method. Sixty-three stool samples with known fat content (range 4.79-292.5 mg/g), 24 samples with known nitrogen content (range 5.36- 19.38 mg/g), and 24 samples with known water concentration (range 60.1 -82.22%) served for calibration, A further 69 samples were analyzed to validate the procedure. Results: There was a satisfactory correlation between the measurements produced by near-infrared reflectance spectr oscopy and those produced by standard methods: fat r = 0.97; nitrogen r = 0.94 water r = 0.96. Conclusions: Near-infrared reflectance spectr oscopy appears to be a reliable, simple, and rapid method of measuring different fecal components-as precise and accurate as the standard me thods. Stool samples should be analyzed immediately after collecting o r stored only a few days before analyzing. (C) 1997 Lippincott-Raven P ublishers.