DETECTION OF CHROMOSOME ANEUPLOIDIES BY USING FLUORESCENCE IN-SITU HYBRIDIZATION ON FETAL TISSUE-SECTIONS

The use of in situ hybridization to detect and characterize chromosome anomalies on cytogenetic preparations is now largely applied in clini cal laboratories. Its use in embryofetopathology on formalin-fixed and paraffin-embedded tissues, when an aneuploid condition is suspected b ut a routine chromosomal analysis is not possible was assessed in the present study. Control values of hybridization signals obtained after different enzymatic digestion protocols have been established on norma l fetal tissues. Tissue conservation and fetal age as well as other pa rameters have also been analysed. A successfully hybridization has bee n achieved in most cases. Failure of hybridization has observed in spe cimens associated with extensive tissular lysis. In conclusion, the ap plication of in situ hybridization on fetal tissues is very useful to detect chromosome anomalies in embryofetopathology.