MICROFLUORIMETRIC IDENTIFICATION AND ASSESSMENT OF PHYSIOLOGICAL-STATE OF LYMPHOCYTES AND NEUTROPHILS IN THE WHOLE NATIVE BLOOD BY MEANS OFFLUORESCENT PROBE-CATION DSM - CLINICAL AND EXPERIMENTAL-DATA

Microfluorimetric identification and assessment of physiological state of lymphocytes and neutrophils in the whole native blood by means of fluorescent probe-cation DSM: clinical and experimental data. G. I. Mo rozova, N. A. Onischenko, I. G. Orzhekhovskaya, O. V. Polosina, F. Kh. Bazieva, O. V. Baukina. Research Institute of Transplantology and Art ificial Organs, Moscow, Russia. The authors present an original method of identification of lymphocytes and neutrophils and assessment of th eir mitochondrial activity in the whole native blood smear. The techni que is based on the use of vital potential-sensitive fluorescent probe -cation (4-p-dimethylaminostyryl-1-methylpyridinium (DSM) and microflu orimetric technique. Clinical trial of the technique demonstrates: the re are two phases of an increase in the mean intensity of mitochondria l fluorescence intensity in the blood of patients with chronic hepatic insufficiency before and after hemoperfusion through fragments of xen ospleen or xenoliver (immediate phase and delayed phase): these phases reflect different mechanisms of lymphocyte activation (a direct effec tor action of splenic organ-specific factors on lymphocyte subpopulati on, antigenic reaction of the body to xenotissues). The detected high reactivity of mitochondrial complex in lymphocytes dependent on their functional activity warrants informative value and short duration of t he technique for estimation of changes in physiological state of lymph ocytes in native blood in different impacts in vitro and in vivo. The method is promising for follow-up control of the treatment efficacy.