Xy. Fu et al., SEPARATION AND DETERMINATION OF ORGANIC-ACIDS IN FOODS BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION, Gaodeng xuexiao huaxue xuebao, 18(9), 1997, pp. 1453-1455
Capillary electrophoresis(CE) with indirect UV detection method for th
e separation and determination of several organic acids commonly found
in foods were developed. Effects of pH, background electrolytes and e
lectroosmotic flow modifiers on separation were investigated. The carr
ier electrolyte pH played an important role in the separation of organ
ic acids. All analytes could be separated in one run by performing CE
with 5.0 mmol/L phthalate-3.0 mmol/L dodecyl trimethyl ammonium chlori
de (DTAC) at pH=7.0 within 6 min. The reproducibility (RSD) of the met
hod is less than 0.17% for migration time and 4.2% for the peak area (
n=8). Linearity was observed more than one order between 1 and 80 mg/L
. The detection limits are obtained from 0.5 to 5.0 mg/L. A variety of
matrix samples such as wine, juices and soy sauce were determined by
the method. The recoveries were in the range of 95%similar to 102%.