SYNTHESIS OF HIGH-EFFICIENCY PACKING AND USING FOR RECOMBINANT HUMAN INTERFERONS SEPARATION IN HIGH-PERFORMANCE HYDROPHOBIC INTERACTION CHROMATOGRAPHY

The synthesis of packing of oxygen-ethane as end-ligand bonded to sili ca and separation for recombinant human interferons(rhIFN) in high-per formance hydrophobic interaction chromatography has been studied in th is paper. It was showed that the packing synthesized was not only suit able for separating standard proteins, but also for different rhIFNs, i.e., rhIFN-alpha(A) expressed in yeast, rhIFN-alpha(A) and rhIFN-gamm a in E. coli. The purify of three rhIFNs purified by one-step HPHIC, w hich rhIFN-gamma expressed in E. coli was recognized as 80%, rhIFN-alp ha(A) expressed in yeast as 70%, and rhIFN-alpha(A) expressed in E. co li as 30%, was more than that of other chromatography besides affinity chromatography.