Authors
Citation
Ka. Brayton et al., DEVELOPMENT OF THE OPGUN(TM) FOR BOMBARDMENT OF ANIMAL-TISSUES, Onderstepoort journal of veterinary research, 64(2), 1997, pp. 153-156
Citations number
9
Categorie Soggetti
Veterinary Sciences
Journal title
ISSN journal
00302465
Volume
64
Issue
2
Year of publication
1997
Pages
153 - 156
Database
ISI
SICI code
0030-2465(1997)64:2<153:DOTOFB>2.0.ZU;2-R
Abstract
A simple and inexpensive particle-bombardment device, the OPgun(TM), w
as constructed for the delivery of DNA into animal tissues. This devic
e is based on the particle-inflow gun first described for plant-cell t
ransfection. The delivery of tungsten particles into the epidermis of
the mouse ear, without the use of vacuum and without causing damage to
the tissue, was demonstrated. The system was also shown to be capable
of inducing antibodies to a foreign gene in mice.