TRANSFORMING GROWTH FACTOR-BETA(2) INHIBITS INTERLEUKIN 1-BETA-INDUCED EXPRESSION OF INDUCIBLE NITRIC-OXIDE SYNTHASE IN RAT RENAL MESANGIALCELLS

Citation
D. Kunz et al., TRANSFORMING GROWTH FACTOR-BETA(2) INHIBITS INTERLEUKIN 1-BETA-INDUCED EXPRESSION OF INDUCIBLE NITRIC-OXIDE SYNTHASE IN RAT RENAL MESANGIALCELLS, Inflammation research, 46(9), 1997, pp. 327-331
Citations number
31
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
ISSN journal
10233830
Volume
46
Issue
9
Year of publication
1997
Pages
327 - 331
Database
ISI
SICI code
1023-3830(1997)46:9<327:TGFII1>2.0.ZU;2-I
Abstract
Objective and Design: We have investigated whether transforming growth factor beta(2) (TGF-beta(2)) influences the expression of inducible n itric oxide synthase (iNOS). Material: Rat renal mesangial cells expos ed to the inflammatory cytokine interleukin 1 beta (IL-1 beta). Result s: Addition of TGF-beta(2) dose-dependently suppresses IL-1 beta-induc ed nitrite formation. Western and Northern blot analyses of mesangial cell extracts reveal that the inhibition of IL-1 beta-induced nitrite formation by TGF-beta(2) is due to decreased iNOS protein and iNOS mRN A steady state levels. Reduction of iNOS mRNA levels is due to decreas ed transcriptional activity of the iNOS gene under the action of TGF-b eta(2) as shown by nuclear run on experiments. Conclusions: TGF-beta(2 ) is a potent inhibitor of iNOS expression acting by reducing the tran scriptional activity of the iNOS gene.