FINE MAPPING OF A REGION OF COMMON DELETION ON CHROMOSOME ARM 10P IN HUMAN GLIOMA

Allelic loss on chromosome 10 is a frequent event in high grade glioma s. Earlier studies have shown that in most cases a complete copy of ch romosome 10 is lost in the tumor. To define more accurately and specif ically the region of common deletion on chromosome arm 10p, we have sc reened a large series of gliomas for allelic losses that exclusively a ffect this part of the chromosome. Allelic loss profiles were determin ed for 127 gliomas, including 118 astrocytomas of various malignancy g rades. Seventeen tumors displayed loss of part of chromosome 10. in th ree of these, only chromosome arm 10p sequences were lost. The interva l between loci D10S559 and D1OS1435 in 10p15, with a length of approxi mately 800 kilobase pairs, was commonly deleted in the latter tumors, suggesting that this region may harbor a tumor suppressor gene importa nt in glioma tumorigenesis. Comparison of the allelic loss profiles in the low and high grade astrocytomas revealed that astrocytoma progres sion is associated with increased loss of chromosome 10 sequences. (C) 1997 Wiley-Liss, Inc.