T. Misteli et Dl. Spector, APPLICATIONS OF THE GREEN FLUORESCENT PROTEIN IN CELL BIOLOGY AND BIOTECHNOLOGY, Nature biotechnology, 15(10), 1997, pp. 961-964
The recent emergence of an autofluorescent protein, the green fluoresc
ent protein (GFP), has opened the door for the convenient use of intac
t living cells and organisms as experimental systems in fields ranging
from cell biology to biomedicine. We present an overview of some of t
he major applications of GFP, namely its use in protein tagging and in
monitoring gene expression as well as its potential in a variety of b
iological screens.