E. Rubinson et al., SEROLOGICAL DETECTION OF GRAPEVINE VIRUS A USING ANTISERUM TO A NONSTRUCTURAL PROTEIN, THE PUTATIVE MOVEMENT PROTEIN, Phytopathology, 87(10), 1997, pp. 1041-1045
Grapevine virus A (GVA) is implicated in the etiology of the rugose wo
od disease. The coat protein (CP) and the putative movement protein (M
P) genes of GVA were cloned and expressed in Escherichia coli and used
to produce antisera. Both the CP and the MP were detected with their
corresponding antisera in GVA-infected Nicotiana benthamiana. The MP w
as first detected at an early stage of the infection, 6 to 12 h after
inoculation, and the CP was detected 2 to 3 days after inoculation. Th
e CP and MP were detected by immunoblot analysis in rugose wood-affect
ed grapevines. The MP could be detected in GVA-infected grapevines tha
t tested negative for CP, both with CP antiserum and with a commercial
ly available enzyme-linked immunosorbent assay kit. The study shows th
at detection of the nonstructural MP may be an effective means for ser
ological detection of GVA infection in grapevines.