CYTOSAMINOMYCINS, NEW ANTICOCCIDIAL AGENTS PRODUCED BY STREPTOMYCES SP KO-8119 .1. TAXONOMY, PRODUCTION, ISOLATION AND PHYSICOCHEMICAL AND BIOLOGICAL PROPERTIES

Streptomyces amakusaensis KO-8119, a soil isolate, was found to produc e a series of new anticoccidial compounds. Four active compounds, desi gnated as cytosaminomycins A, B, C and D, were isolated from the ferme ntation broth of the producing strain by solvent extraction, silica ge l column chromatography and preparative HPLC. Cytosaminomycins inhibit ed the growth of Eimeria tenella in an in vitro assay system using pri mary chicken embryonic cells as a host. No schizont in the cells was o bserved at concentrations ranging from 0.3 to 0.6 mu g/ml for cytosami nomycins A, B and C, and at 2.5 mu g/ml for cytosaminomycin D.