INTERACTION OF TROUT HEMOGLOBIN WITH H2O2 - A CHEMILUMINESCENCE STUDY

Erythrocytes from trout Salmo irideus are characterized by four differ ent hemoglobin components (HbI, HbII, HbIII and HbIV), HbI and HbIV be ing predominant, In this study we describe the interaction between tro ut hemoglobin (HbI and HbIV) and H2O2 using a chemiluminescence assay. Our data show that the reaction of hemoglobins with H2O2 produces a t ime-limited and significant increase of chemiluminescence signal. The half-life of the decay of this chemiluminescence signal was characteri stic for each type of hemoglobin used. These results indicate the form ation of excited molecules related to the interaction between trout he moglobin and H2O2. (C) 1997 John Wiley & Sons, Ltd.