APPLICATION OF THE BICINCHONINIC METHOD OF ASSAY FOR THE REDUCING SUGARS TO DETERMINE CARBOXYMETHYLCELLULASE ACTIVITY OF CELLULASES USING AMICROPLATE READER

Authors
ZOROV IN DUBASOVA MY SINITSYN AP GUSAKOV AV MITCHENKO AA BARAZNENOK VA GUTYERRES B POPOVA NN
Citation
In. Zorov et al., APPLICATION OF THE BICINCHONINIC METHOD OF ASSAY FOR THE REDUCING SUGARS TO DETERMINE CARBOXYMETHYLCELLULASE ACTIVITY OF CELLULASES USING AMICROPLATE READER, Biochemistry, 62(7), 1997, pp. 704-709
Citations number
7
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
62
Issue
7
Year of publication
1997
Pages
704 - 709
Database
ISI
SICI code
0006-2979(1997)62:7<704:AOTBMO>2.0.ZU;2-1
Abstract
A highly sensitive and rapid method of assay of the carboxymethylcellu lase activity using ELISA plates was developed. The method includes hy drolysis of carboxymethylcellulose solution with cellulase preparation s and subsequent determination of reducing sugars concentration in the wells of the plate by the bicinchoninic method. Comparison of the Som ogyi-Nelson method with the bicinchoninic method indicates that the la tter is more rapid and requires less reagents for serial analyses.