Va. Baraznenok et al., COMPARISON OF CELLULASE ACTIVITY ASSAYS WITH STAINED AND UNSTAINED CELLULOSE DERIVATIVES, Biochemistry, 62(7), 1997, pp. 760-766
Methods of cellulase activity assay were compared with carboxymethylce
llulose (CMC) stained with Remazol Brilliant Blue R, cross-linked hydr
oxyethylcellulose stained with azurin, and unstained CMC as the substr
ates and preparations of various cellulase complexes from different pr
oducers and purified cellulases. Generally, there is no correlation be
tween specific endo-1,4-beta-glucanase activity assayed with viscosime
try and activity assayed with stained substrates. Effects of pH and te
mperature obtained with CMC and stained CMC can differ significantly.
The observed differences may be due to variable specificity of cellula
ses towards stained and unstained cellulose derivatives.