ASSIGNMENT OF THE HUMAN GENE ENCODING EUKARYOTIC INITIATION-FACTOR 4E(EIF4E) TO THE REGION Q21-25 ON CHROMOSOME-4

We recently cloned genomic sequences containing the promoter region fo r the messenger RNA cap binding protein (eIF4E). As the rate-limiting step in translation, eukaryotic initiation factor 4E is important in c ellular growth control. Using oligonucleotide primers specific for the promoter region in polymerase chain reactions (PCR), we amplified the human gene in a chromosome 4-specific human/rodent somatic cell panel . This panel mapped single copy genomic sequences for eIF4E in the reg ion 4q21 to 4q25.