Mt. Hochereaudereviers et C. Perreau, INDUCED-DIFFERENTIATION OF OVINE FETAL GONOCYTES AFTER GRAFTING IN THE SCROTUM OF NUDE-MICE, Reproduction, nutrition, development, 37(4), 1997, pp. 469-476
This work was designed to elucidate, in foetal ovine testis, whether t
he reason why gonocytes do not differentiate into spermatogonia and do
not initiate spermatogenesis is due to inadequate foetal environment
(temperature and/or hormonal balance) or if somatic testicular cell pr
oliferation is a prerequisite for initiation of male meiosis. The deve
lopment and differentiation of gonocytes were analysed by grafting 60-
day-old foetal ovine testes into the scrotum of immunotolerant adult N
ude mice. Forty days after grafting, nine of the ten grafted testes ha
d survived but had not increased in weight as compared to 60-day-old t
estes. Moreover, only one third of the graft was occupied by testicula
r tissue, in which the relative proportions of intertubular tissue and
sex cords were not altered when compared with those of normal foetal
testes. The remainer of the graft was occupied by teratoma. The total
number of Leydig (-80%) cells, Sertoli (-66%) cells, gonocytes (-90%)
and the total length of sex cords (-63%) per grafted testis were alway
s significantly reduced (P < 0.02), whereas the sex cords were signifi
cantly increased in diameter (+36%; P = 0.02) as compared to those of
non-grafted 60-day-old foetuses. However, in seven out of the nine tes
tes, type A spermatogonia were obtained and in two of the seven a few
type B or leptotene primary spermatocytes could be observed. The graft
ing of foetal testis in an adult scrotum induces differentiation of go
nocytes into spermatogonia, independently of proliferation of Sertoli
and Leydig cells.