GENETIC FINE LOCALIZATION OF THE ARRESTIN (S-ANTIGEN) GENE 4-CM DISTAL FROM D2S17A

Arrestin is a component of the light transduction cascade that takes p lace in the outer segment of retinal rods. In situ hybridization and l inkage analysis have localized the arrestin gene to a region of 50 cM between CRYG alpha and D2S23/D2S55 on chromosome 2q24-37. We have perf ormed pairwise and multipoint linkage analysis between arrestin and fo ur highly polymorphic markers from this region. The results indicate t ight linkage between the gene and the microsatellite D2S172 (Z(max) = 9.25 at theta = 0.038). This fine localization of the gene should prov ide a useful tool for cosegregation analyses involving the arrestin ge ne.