SUBLOCALIZATION OF A LOCUS AT 3P21.3-23 PREDISPOSING TO HEREDITARY NONPOLYPOSIS COLON-CANCER

Hereditary nonpolyposis colon cancer (HNPCC) is a heterogeneous diseas e caused by at least three different genes on chromosomes 2 and 3, and one or more additional chromosomes. We used 19 dinucleotide markers i n order to sublocalize further the 3p locus linked to HNPCC, and to or der the markers into one map resulting in a panel of markers suitable for linkage studies. Human chromosome 3 mouse hybrids were used to det ermine the chromosomal position of the markers. Haplotype analysis in two families where the disease is linked to chromosome 3p21-23 was use d in order to sublocalize further the region in which the gene is loca ted. Based on our results, the gene has now been localized to the regi on 3p21.3-23.