ROLES OF THE PAS1 AND PAR2 GENES IN DETERMINATION OF THE UNIQUE, INTERMEDIATE SUSCEPTIBILITY OF BALB CBYJ MICE TO URETHANE-INDUCTION OF LUNG CARCINOGENESIS - DIFFERENTIAL-EFFECTS ON TUMOR MULTIPLICITY, SIZE AND KRAS2 MUTATIONS/

The C3H/HeJ (C3H), A/J and BALB/cByJ (BALB) mouse strains are respecti vely resistant, sensitive and intermediate regarding the induction of lung tumors by urethane. The phenotypic difference between C3H and A/J is largely determined by the Pas1 (Pulmonary adenoma susceptibility 1 ) gene an chromosome 6, the A/J allele of which dominantly increases t he tumor burden. We recently found Chat BALB mice possess a unique lun g tumor resistance gene on chromosome 18, designated Par2 (Pulmonary a denoma resistance 2), which partially, but dominantly suppresses the s ensitive phenotype of A/J mice (Oncogene 13: 1599-1604, 1996). It has, however, remained unclear why BALB mice carrying the Par2 gene are si gnificantly more sensitive to urethane-induced lung carcinogenesis tha n C3H mice that have no dominant lung tumor resistance genes. In the p resent study, using (C3H x BALB)F-1 x C3H backcross mice treated with urethane, we demonstrated that BALB mice possess the disease allele of the Pas1 gene despite their 15-fold more resistance relative to A/J m ice (LOD = 22.6). The BALB Par2 allele only significantly reduced the mean lung tumor multiplicity (LOD=4.4) in the backcross population car rying the BALB allele of Pas1, indicating that the intermediate BALB p henotype may at least in part be the result of interactions between th ese two dominant genes. While the BALB Pas1 allele increased both the mean multiplicity and size of lung tumors, the BALB Par2 allele affect ed only the mean tumor multiplicity, implying that they are involved i n different stages of multi-step lung carcinogenesis. In addition, we found that 68% of lung tumors from the BALB Pas1-positive backcross mi ce contained activating point mutations of the KRas2 oncogene, tightly linked to the Pas1 locus, whereas these genetic alterations were abse nt in tumors from BALB Pas1-negative mice. The Pal2 genotype exhibited no effect on this parameter. Since the activating point mutations mer e observed exclusively in the BALB allele as already reported with lun g tumors in (C57BL/6J x BALB/cJ)F-1 mice, BALB Pas1 or possibly Kras2 itself may confer selective growth advantage on the affected urethane- initiated lung lesions.