DIAGNOSIS OF PREMATURE RUPTURE OF THE MEMBRANES BY THE IDENTIFICATIONOF ALPHA-FETO-PROTEIN IN VAGINAL SECRETIONS

Premature rupture of membranes (FROM) is sometimes difficult to diagno se. This report proposes the use of alpha-fete-protein (AFP) values in vaginal secretions for diagnostic tests. Our investigation took place in two separate phases. The first phase validated the AFP test using an immuno-enzymatic assay method and determined a threshold value from a sample of 167 female patients (Group 1: 133 patients with an extrem ely low probability of FROM, and Group 2: 54 patients with confirmed F ROM). In the second phase the test was applied to a sample of 145 fema le patients (Group 3) with suspected, but unconfirmed, FROM. Results f rom alpha-fete-protein (AFP) evaluation were compared with data obtain ed from clinical, echographic and other tests. The positive/negative t hreshold adopted was an AFP concentration of 30 mu g/l. For the two fi rst groups, 1 and 2, sensitivity and specificity was in the 98% to 99% confidence level. For Group 3, sensitivity and specificity results at the 30 mu g/l threshold value were 94.5% and 95.4% respectively. Quan titative measurement of AFP determined by immuno-enzymatic assay of va ginal secretions with a threshold of 30 mu g/l is a reliable, simple a nd rapid diagnostic test. Results obtained are significantly better th an the measurement of pH, the determination of prolactin, and more pra ctical than diamino-oxidase (DAO) assays.