INFLUENCE OF HYALURONIC-ACID OR PHORBOL 12-MYRISTATE 13-ACETATE ON THE MIGRATION CAPACITY OF A MURINE LYMPHOMA CELL-LINE (EB) AND ITS METASTATIC VARIANT (ESB)

The in vitro migration of two murine T cell. lymphoma cell lines (Eb a nd ESb) was studied employing a three-dimensional collagen matrix and time-lapse video recording. In the highly metastatic cell line ESb, wh ich had a low spontaneous locomoting activity, migration could clearly be stimulated by hyaluronic acid (HA) whereas only a small increase w as found after incubation with phorbol myristate acetate (PMA). The ob served stimulation could be attributed to an increase in recruitment o f locomoting cells and not to changes in migration parameters of motil e individual cells such as percentage of time locomoting, velocity or distance migrated. Incubation of the low metastatic cell line Eb with RA led to a decrease in migration but blocking of CD44, the principle ligand for HA, by preincubation with an anti-CD44 mAb (KM114), followe d by HA exposure increased the locomoting activity significantly. The effect was based on both an increase in recruitment as well as in all migration parameters regarding motile individual Eb cells. (C) 1997 El sevier Science Ireland Ltd.