UREASE IMMOBILIZED ON NYLON - PREPARATION AND PROPERTIES

Authors
ANITA A SASTRY CA HASHIM MA
Citation
A. Anita et al., UREASE IMMOBILIZED ON NYLON - PREPARATION AND PROPERTIES, Bioprocess engineering, 17(4), 1997, pp. 241-245
Citations number
30
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Bioprocess engineering → ACNP
ISSN journal
0178515X
Volume
17
Issue
4
Year of publication
1997
Pages
241 - 245
Database
ISI
SICI code
0178-515X(1997)17:4<241:UION-P>2.0.ZU;2-#
Abstract
Urease (EC 3.5.1.5) was covalently attached through glutaraldehyde to partially hydrolysed nylon 6/6 tubes. The highest activity of immobili zed enzyme was obtained at 65 degrees C and pH 6.5, while the optimum temperature for free urease was found to be 25 degrees C. Immobilized urease showed an improved thermal stability in comparison to free urea se. It retained 76% of the original activity after 60 days when stored at 4 degrees C and 78% of the activity after 5 repeated uses.