INFLUENCE OF CADMIUM ON SOLUBLE CARBOHYDRATES, FREE AMINO-ACIDS, PROTEIN-CONTENT OF IN-VITRO CULTURED LUPINUS-ALBUS

The objective of this study was to evaluate the consequences of Cd str ess on soluble carbohydrates, free amino acids, proteins, CO2 fixation , stomatal conductance, intercellular CO2 and water potential of white lupin (Lupinus albus L. cv. lublanc) in vitro cultures. Plants were g rown for 15 days in axenic condition in medium containing variable CdN O3 concentrations (0, 0.01, 0.1, 1, 10, 100 mu M Cd). Cd treatment did not cause significant dry weight differences between Cd-treated and c ontrol plants except above 0.1 mu M Cd. At lower Cd concentrations, to tal soluble carbohydrate and total free amino acid of Cd-treated plant s increased, but decreased above 0.1 mu M Cd. In contrast raffinose, m annose and hydroxyproline levels increased with Cd concentration. (C) 1997 Elsevier Science Ireland Ltd.