EFFECTS OF PROTEIN-KINASE INHIBITORS ON THE CELL MOTILITY STIMULATED BY AUTOCRINE MOTILITY FACTOR

Preincubation of Dunn osteosarcoma cells for 1 h with both 100 nM of s taurosporine and 10 mu g/ml of genistein resulted in a significant dec rease in the motility stimulated by autocrine motility factor (AMF), w hereas these reagents did not affect the basal motility and proliferat ion at these concentrations. The effect of the agents on the stimulate d motility was both dose- and time-dependent. The motility stimulated by the anti-AMF receptor mAb was also inhibited. In contrast, H-8 had a negligible effect upon the stimulated motility. These data suggest t hat both kinase C and tyrosine kinase play a role in AMF-stimulated ce ll motility, while protein kinase A, which is selectively associated w ith the adenylate cyclase pathway, may not be required for the stimula tion.