A NOTE ON THE PHYSIOLOGICAL BACKGROUND OF THE ETHYLENE-OXIDE ADDUCT 7-(2-HYDROXYETHYL)GUANINE IN DNA FROM HUMAN BLOOD

Authors
BOLT HM LEUTBECHER M GOLKA K
Citation
Hm. Bolt et al., A NOTE ON THE PHYSIOLOGICAL BACKGROUND OF THE ETHYLENE-OXIDE ADDUCT 7-(2-HYDROXYETHYL)GUANINE IN DNA FROM HUMAN BLOOD, Archives of toxicology, 71(11), 1997, pp. 719-721
Citations number
11
Categorie Soggetti
Toxicology
Journal title
Archives of toxicology → ACNP
ISSN journal
03405761
Volume
71
Issue
11
Year of publication
1997
Pages
719 - 721
Database
ISI
SICI code
0340-5761(1997)71:11<719:ANOTPB>2.0.ZU;2-L
Abstract
A newly developed high-performance liquid chromatography (HPLC) method involves derivatization with phenylglyoxal and fluorescence detection , using 7-methylguanine as internal standard. The physiological backgr ound of the adduct 7-(2-hydroxyethyl)guanine in DNA isolated from huma n blood was determined by this method. In five persons the range of th e adduct was between 2.1 and 5.8 pmol/mg DNA (mean 3.2). This finding is consistent with previous data obtained by others, using different a nalytical methods, and points to an intrinsic carcinogenic risk due to endogenous ethylene oxide.