IN-SITU HYBRIDIZATION MAPPING OF GENOMIC CLONES FOR 5 HUMAN RESPIRATORY-CHAIN COMPLEX-I GENES

Using human and bovine short cDNA sequences as probes we screened huma n cosmid and P1 libraries for components of the complex I multi-subuni t enzyme of oxidative phosphorylation. We isolated genomic recombinant s encoding cI-B8 (gene NDUFA2), cI-B14 (gene NDUFA6), cI-B14.5a (gene NDUFA7), cI-ASHI (gene NDUFB8) and cI-23kD (gene NDUFS8). Genomic vers ions of these genes have not been previously cloned in the human altho ugh they are represented as anonymous entries in public cDNA databases . By using the derived genomic clones for in situ hybridisation studie s we determined the following chromosome locations: NDUFA2, 5q31; NDUF A6, 21q22; NDUFA7, 20p13; NDUFB8, 12q21; NDUFS8, 3q28.