EXPERIMENTAL ASSESSMENT OF THE DETECTION LIMIT OF GENOMIC AMPLIFICATION BY COMPARATIVE GENOMIC HYBRIDIZATION CGH

Authors
PARENTE F GAUDRAY P CARLE GF TURCCAREL C
Citation
F. Parente et al., EXPERIMENTAL ASSESSMENT OF THE DETECTION LIMIT OF GENOMIC AMPLIFICATION BY COMPARATIVE GENOMIC HYBRIDIZATION CGH, Cytogenetics and cell genetics, 78(1), 1997, pp. 65-68
Citations number
9
Categorie Soggetti
Cell Biology","Genetics & Heredity
Journal title
Cytogenetics and cell genetics → ACNP
ISSN journal
03010171
Volume
78
Issue
1
Year of publication
1997
Pages
65 - 68
Database
ISI
SICI code
0301-0171(1997)78:1<65:EAOTDL>2.0.ZU;2-8
Abstract
Artificial amplicons of known size, constructed by use of YACs featuri ng human 8p12 and 12q13, were analyzed by comparative genomic hybridiz ation (CGH). A minimum of 15 Mb of overrepresented DNA sequences could be detected. The sensitivity is (1) not dependent on the chromosome s ite and (2) related to the size of the amplicon, decreasing with decre asing size.