Ok. Baskurt et al., IN-VITRO EFFECTS OF IN-VIVO ACTIVATED LEUKOCYTES ON RBC FILTERABILITYAND LIPID-PEROXIDATION, Clinical hemorheology, 14(4), 1994, pp. 591-596
Activated leukocytes are known to generate reactive oxygen species whi
ch may attack other cells in their surroundings. On the other hand, rh
eological properties of red blood cells (RBC) are affected by oxidant
attack.This study was designed to determine the influence of activated
leukocytes on RBC rheology in vitro. Activated leukocytes were isolat
ed from the Guinea pigs with an induced E. Coli sepsis and incubated w
ith RBC obtained from normal animals. After two hours of incubation wi
th activated leukocytes, RBC deformability indices were increased 1.23
+/- 0.05 (Mean+/-Standart error) times (compared to the control RBC s
uspensions) while incubation with leukocytes obtained from healthy ani
mals increased deformability indices 1.12 +/- 0.03 times. Thiobarbitur
ic acid reactive substances as a measure of lipid peroxidation were in
creased 2.95 +/- 0.36 times in the RBC suspensions incubated with acti
vated leucocytes, but 1.70 +/- 0.10 times in suspensions incubated wit
h normal leukocytes. These results suggest that RBC mechanical propert
ies might be affected by activated leukocytes and possibly an oxidativ
e mechanism plays role in this interaction.