ENZYMATIC PRODUCTION OF GLUCOSE-1-P USING PHOSPHORYLASE FROM POTATO STARCH WASTE-WATER

Potato starch wastewater industry contains-a considerable amount of nu trients and enzymatic activity that could be recovered, purified or re used in the process before being discharged. In this investigation the effect of wastewater purity and the ratio of phosphate-starch concent ration on glucose-l-P production was evaluated. Three purification met hods were applied to wastewater or potato juice: centrifugation, therm ic treatment and precipitation. Preliminary results show that raw cent rifuged potato juice yielded 27% more glucose-1-P than heated or preci pitated juice, due to the presence of both a-amylase and phosphorylase in the raw centrifuged juice. Also, a maximum 80% yield was obtained using a phosphate:starch concentration ratio of 8.5.