PRODUCTION OF RECOMBINANT PROTEINS BY METHYLOTROPHIC YEASTS

The methylotrophic yeasts Hansenula polymorpha, Pichia pastoris and Ca ndida boidinii have been developed as production systems for recombina nt proteins. The favourable and most advantageous characteristics of t hese species have resulted in an increasing number of biotechnological applications. As a consequence, these species - especially H. polymor pha and P. pastoris - are rapidly becoming the systems of choice for h eterologous gene expression in yeast, Recent advances in the developme nt of these yeasts as hosts for the production of heterologous protein s have provided a catalogue of new applications, methods and system co mponents.