GENE-EXPRESSION SYSTEMS IN THE DEVELOPMENT OF HIGH-THROUGHPUT SCREENS

Recent advances in the development of combinatorial automated chemical synthesis, robotic sample handling, and data collection and analysis have significantly increased the number of compounds available for scr eening against potential therapeutic targets. The implementation of hi ghly sensitive in vitro biochemical and cell-based high-throughput scr eening assays is essential to facilitate the rapid identification of s elective and potent lead molecules from compound libraries. The abilit y to easily produce functional proteins in sufficient quantities for i n vitro biochemical assays and to devise useful cell-based systems is dependent on the successful application of a variety of gene expressio n systems.