IMMUNOHISTOLOGICAL ASSESSMENT OF FIBRIN DEPOSITION AND THROMBUS FORMATION IN CANINE MAMMARY NEOPLASIA

A commercially available monoclonal antibody against human fibrin was used to detect fibrin in canine formalin-fixed, paraffin was-embedded tissue by applying a slightly modified alkaline phosphatase anti-alkal ine phosphatase (APAAP) technique. Twenty-eight mammary tumours from s ix bitches were examined for the presence of fibrin. Thrombi and extra vascular fibrin deposits were detected in 15 tumours (12 complex adeno carcinomas, one adenocarcinoma; two solid carcinomas), and a single th rombus was detected in one adenoma; 12 tumours (three adenomas, one co mplex adenoma, four complex adenocarcinomas and four adenocarcinomas) did not show any staining reaction. (C) 1997 W.B. Saunders Company Lim ited.