Pgm. Zweipfenning et al., TOXICOLOGICAL ANALYSIS OF WHOLE-BLOOD SAMPLES BY MEANS OF BOND-ELUT CERTIFY COLUMNS AND GAS-CHROMATOGRAPHY WITH NITROGEN-PHOSPHORUS DETECTION, Journal of chromatography, 674(1-2), 1994, pp. 87-95
The application of Bond-Elut Certify solid-phase extraction columns to
the systematic toxicological analysis of whole blood was evaluated. T
he reproducibility of the extraction was tested with thirteen drugs va
rying in physico-chemical properties. Analysis was performed with capi
llary gas chromatography with nitrogen-selective detection. The recove
ries were reproducible, as long as other limiting factors, e.g., chrom
atographic behaviour or volatility, do not play a significant role. Th
e effect of limiting chromatographic behaviour was studied in more det
ail with the more sensitive mass spectrometry with selected-ion monito
ring after converting the extracted morphine into its ditrimethylsilyl
derivative.