Mc. Gennaro et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF FOOD COLORS AND ITS RELEVANCE IN FORENSIC CHEMISTRY, Journal of chromatography, 674(1-2), 1994, pp. 281-299
The forensic interest in the use of food colours is related to health
rights and safeguard of the consumer, as established in national legis
lations. The regulations related to food dyes consider the health of h
uman beings and the adulteration of foodstuffs and economic needs conn
ected with quantitative restrictions on imports. Efficient analytical
methods are required for evaluating toxicity and authenticity or adult
eration, in order (a) to determine whether there are synthetic dyes pr
esent in foods and whether they are permitted, (b) to determine the le
vels, (c) to confirm the absence of added dyes in foods where they are
not declared and (d) to check on the stability of dyes during process
ing and storage. Recent HPLC methods for the identification and determ
ination of natural and synthetic dyes and of carcinogenic amines conta
ined as impurities in synthetic dyes are reviewed.