REGULATION OF DECIDUAL CELL AND CHORION CELL PRODUCTION OF INTERLEUKIN-10 BY PURIFIED BACTERIAL PRODUCTS

PROBLEM: To determine whether cultured human decidual cells and chorio n cells produce interleukin-10 (IL-IO) after incubation with purified bacterial products. METHOD OF STUDY: Decidual cell cultures and chorio n cell cultures were established by standard techniques. With confluen ce, monolayers of each culture were incubated with purified bacterial products, including various concentrations of lipopolysaccharide (LSI, lipid A, and lipoteichoic acid (LTA) for 16 hr in quadruplicate. Cult ure supernatants were collected and assayed for immunodetectable IL-IO by enzyme-linked immunoadsorbent assay (ELISA). RESULTS: Bath decidua l cell cultures and chorion cell cultures produced significant quantit ies of IL-10 after stimulation with LPS, lipid A, and LTA. Cultures of decidual cells produced more IL-IO than did chorion cell cultures. CO NCLUSIONS: Our data indicate that both maternal decidual cells and fet ally derived chorion cells can produce IL-10 after incubation with bac terial virulence Factors. This finding contrasts with our previous fin dings in which chorion cells did not produce IL-10 after stimulation w ith IL-1 beta. suggesting that chorion cell production after incubatio n with bacterial products is independent of IL-1 beta. We speculate th at the contribution of anti-inflammatory IL-10 production by human ges tational tissues to the inflammatory process in these tissues may be o vercome or abrogated by the pro-inflammatory process.