OPTIMIZED DIFFERENTIAL DISPLAY AND REAMPLIFICATION PARAMETERS FOR SILVER STAINING

Citation
S. Gottschlich et al., OPTIMIZED DIFFERENTIAL DISPLAY AND REAMPLIFICATION PARAMETERS FOR SILVER STAINING, Research communications in molecular pathology and pharmacology, 97(2), 1997, pp. 237-240
Citations number
5
Categorie Soggetti
Pharmacology & Pharmacy",Pathology,Biology
ISSN journal
10780297
Volume
97
Issue
2
Year of publication
1997
Pages
237 - 240
Database
ISI
SICI code
1078-0297(1997)97:2<237:ODDARP>2.0.ZU;2-A
Abstract
Differential display (DD) is a powerful instrument to detect differenc es in gene expression of malignant and normal cells. An optimized silv er staining protocol was used to compare mRNA expression of keratinocy tes and squamous cell carcinoma cells of the head and neck. Optimal co ncentration for upstream and downstream primer was 0.2 mu M. Best prim er concentrations for reamplification were between 40 and 60 pM. With this optimized protocol nearly 50 differentially expressed transcripts have been identified and differential display can be applied safer, e asier, and faster, than by radioactive labeling.