S. Gottschlich et al., OPTIMIZED DIFFERENTIAL DISPLAY AND REAMPLIFICATION PARAMETERS FOR SILVER STAINING, Research communications in molecular pathology and pharmacology, 97(2), 1997, pp. 237-240
Differential display (DD) is a powerful instrument to detect differenc
es in gene expression of malignant and normal cells. An optimized silv
er staining protocol was used to compare mRNA expression of keratinocy
tes and squamous cell carcinoma cells of the head and neck. Optimal co
ncentration for upstream and downstream primer was 0.2 mu M. Best prim
er concentrations for reamplification were between 40 and 60 pM. With
this optimized protocol nearly 50 differentially expressed transcripts
have been identified and differential display can be applied safer, e
asier, and faster, than by radioactive labeling.