CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A TRUNCATED FAMILY-A ALKALINE ENDOGLUCANASE ISOLATED FROM BACILLUS SP. KSM-635

Authors
SHIRAI T YAMANE T HIDAKA T KUYAMA K SUZUKI A ASHIDA T OZAKI K ITO S
Citation
T. Shirai et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A TRUNCATED FAMILY-A ALKALINE ENDOGLUCANASE ISOLATED FROM BACILLUS SP. KSM-635, Journal of Biochemistry, 122(4), 1997, pp. 683-685
Citations number
13
Categorie Soggetti
Biology
Journal title
Journal of Biochemistry → ACNP
ISSN journal
0021924X
Volume
122
Issue
4
Year of publication
1997
Pages
683 - 685
Database
ISI
SICI code
0021-924X(1997)122:4<683:CAPAOA>2.0.ZU;2-W
Abstract
The catalytic domain of an alkaline endo-1,4-beta-glucanase (family A) isolated from Bacillus sp, KSM-635 (M-r=40.2kDa) was crystallized usi ng the hanging drop vapor diffusion method, Two different crystal form s were obtained. Form 2 crystals (trigonal space group R3 with cell di mensions of a=b=111.9 and c=207.1 Angstrom in a hexagonal lattice) wer e found to be more stable than form 1 ones upon X-ray irradiation, A f ull data set for form 2 crystals has been collected up to 3.3 Angstrom resolution.