BEAD-TO-BEAD TRANSFER OF CHINESE-HAMSTER OVARY CELLS USING MACROPOROUS MICROCARRIERS

Citation
S. Ohlson et al., BEAD-TO-BEAD TRANSFER OF CHINESE-HAMSTER OVARY CELLS USING MACROPOROUS MICROCARRIERS, Cytotechnology, 14(1), 1994, pp. 67-80
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
ISSN journal
09209069
Volume
14
Issue
1
Year of publication
1994
Pages
67 - 80
Database
ISI
SICI code
0920-9069(1994)14:1<67:BTOCOC>2.0.ZU;2-0
Abstract
Chinese hamster ovary cells (CHO-K1) were cultivated in macroporous ge latin microcarriers (CultiSpher G and CultiSpher S) in spinner flasks and a 5 1 bioreactor. Near-to-confluent cultures were harvested by bea d-to-bead transfer where intact microcarriers with cells were transfer red from a spinner flask to another spinner flask or to the bioreactor with naked microcarrier beads. Successful bead-to-bead transfer was a chieved in various split ratios. The duration of attachment seemed to be important where the direct contact of beads to each other can be ac hieved by intermittent stirring. Repeated transfers were performed and at least four transfers in spinner flasks were achieved. Two variatio ns of bead-to-bead transfer were performed in the 5 1 bioreactor eithe r by seeding the bioreactor with near-to-confluent beads cultivated in spinner flasks or in situ transfer by adding fresh beads to the biore actor. As in the spinner case, attachment was achieved by intermittent stirring where donor beads were in close proximity to the acceptor be ads. Again successful transfers were obtained as evidenced by the good growth on acceptor beads where cell yields were in the range of 3100- 4500 cells/bead. The results suggest that bead-to-bead transfer of CHO -K1 cells can be easily performed and do provide an alternative route to applications where dissolution techniques may not offer an efficien t solution.