EFFECT OF DIFFERENT CYTOKININS ON AXILLARY SHOOT PROLIFERATION AND ELONGATION OF SEVERAL GENOTYPES OF SEQUOIA SEMPERVIRENS

Authors
Citation
Iw. Sul et Ss. Korban, EFFECT OF DIFFERENT CYTOKININS ON AXILLARY SHOOT PROLIFERATION AND ELONGATION OF SEVERAL GENOTYPES OF SEQUOIA SEMPERVIRENS, In vitro cellular & developmental biology. Plant, 30P(3), 1994, pp. 131-135
Citations number
18
Categorie Soggetti
Developmental Biology","Cytology & Histology","Plant Sciences
ISSN journal
10545476
Volume
30P
Issue
3
Year of publication
1994
Pages
131 - 135
Database
ISI
SICI code
1054-5476(1994)30P:3<131:EODCOA>2.0.ZU;2-P
Abstract
Five genotypes of Sequoia sempervirens were cultured in vitro. Stem se gments of greenhouse-grown plants were disinfested and grown on a Welt er and Skoog (WS) medium without growth regulators for 4 wk. Newly dev eloping shoots from axillary buds were then subcultured onto fresh med ia containing several different cytokinins at a concentration of 5 mu M each per treatment. The following cytokinin treatments were used: be nzyladenine (BA), BA plus adenine hemisulfate, N-benzyl-9(2-tetrahydro pyranyl) adenine (BPA), N6-[2-Isopentenyl]adenine (2ip), kinetin, thid iazuron (TDZ), and zeatin. Each genotype responded differently to test ed cytokinins. The use of zeatin resulted in the highest number of sho ots and the longest shoots for three genotypes of S. sempervirens. In another experiment, shoots from three genotypes were grown on the same basal medium described above and supplemented with zeatin at six diff erent concentrations (2.5, 5, 10, 15, 20, and 30 mu M). For all zeatin concentrations, significant differences among genotypes for shoot pro liferation were observed. When all five genotypes were grown under thr ee concentrations of zeatin (5, 10, and 15 mu M), differences among ge notypes were observed for both shoot proliferation and shoot length. T he influence of the culture medium on the overall micropropagation pro tocol of S. sempervirens is discussed.