PREPARATION AND GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC ANALYSIS OF N-ACETYL-O-TRIMETHYLSILYL DERIVATIVES OF LONG-CHAIN BASE RESIDUES OF NATURAL SPHINGOMYELIN

Authors
OLSSON NU KAUFMANN P DZELETOVIC S
Citation
Nu. Olsson et al., PREPARATION AND GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC ANALYSIS OF N-ACETYL-O-TRIMETHYLSILYL DERIVATIVES OF LONG-CHAIN BASE RESIDUES OF NATURAL SPHINGOMYELIN, Journal of chromatography B. Biomedical sciences and applications, 698(1-2), 1997, pp. 1-8
Citations number
32
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
698
Issue
1-2
Year of publication
1997
Pages
1 - 8
Database
ISI
SICI code
0378-4347(1997)698:1-2<1:PAGMAO>2.0.ZU;2-N
Abstract
A procedure for the preparation of long-chain base residues of egg yol k, bovine milk and bovine brain sphingomyelin was developed. The bases were converted to N-acetyl-O-trimethylsilyl (TMS) derivatives before being submitted to gas chromatography and mass spectrometry. The chrom atographic profile of the milk sample was complex with thirteen peaks, whereas the profiles of brain and egg yolk long-chain bases were simp le and remarkably similar. (C) 1997 Elsevier Science B.V.