EFFECT OF DIFFERENT SURFACTANTS ON THE SEPARATION BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY OF A COMPLEX MIXTURE OF DIPEPTIDES IN URINE OF PROLIDASE-DEFICIENT PATIENTS
R. Grimm et al., EFFECT OF DIFFERENT SURFACTANTS ON THE SEPARATION BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY OF A COMPLEX MIXTURE OF DIPEPTIDES IN URINE OF PROLIDASE-DEFICIENT PATIENTS, Journal of chromatography B. Biomedical sciences and applications, 698(1-2), 1997, pp. 47-57
Citations number
28
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Prolidase deficiency is a severe disorder characterized by massive exc
retion of metabolites with closely related structures. At present, mic
ellar electrokinetic chromatography is the separation method which pro
vides the highest selectivity of structurally similar solutes. However
, the structure of a surfactant can greatly affect the selectivity of
separation depending on factors such as the length of hydrophobic alky
l chain or the nature of the hydrophilic group. Here we investigated t
he effect of three non-ionic and four anionic detergents for obtaining
the best separation conditions for resolving imidodipeptide mixtures.
The effect on resolution of variables such as temperature, surfactant
concentrations and organic solvents was also examined. The greatest r
esolution was obtained at the lowest temperature studied (10 degrees C
) using 50 mM sodium berate, pH 9.3 containing 50 mM pentanesulfonate
and 10% (v/v) methanol. Under these experimental conditions almost all
excreted components were baseline separated and identified. (C) 1997
Elsevier Science B.V.