DETERMINATION OF SAQUINAVIR IN HUMAN PLASMA, SALIVA, AND CEREBROSPINAL-FLUID BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

Authors
HOETELMANS RMW VANESSENBERG M MEENHORST PL MULDER JW BEIJNEN JH
Citation
Rmw. Hoetelmans et al., DETERMINATION OF SAQUINAVIR IN HUMAN PLASMA, SALIVA, AND CEREBROSPINAL-FLUID BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION, Journal of chromatography B. Biomedical sciences and applications, 698(1-2), 1997, pp. 235-241
Citations number
10
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
698
Issue
1-2
Year of publication
1997
Pages
235 - 241
Database
ISI
SICI code
0378-4347(1997)698:1-2<235:DOSIHP>2.0.ZU;2-5
Abstract
A high-performance liquid chromatographic method for the determination of the HIV protease inhibitor saquinavir in human plasma, saliva, and cerebrospinal fluid is described. Saquinavir was extracted from sampl es using C-2 extraction columns prior to ion-pair, reversed-phase high -performance liquid chromatography with ultraviolet detection at 239 n m. The method has been validated over the range of 2.5-4000 ng/ml usin g a 0.6-ml sample volume. This assay has been used for the analysis of saquinavir in plasma and saliva of HIV-1-infected patients. (C) 1997 Elsevier Science B.V.