SPECIFICITY FOR IN-VIVO GRAFT PROLONGATION IN GAMMA-DELTA T-CELL RECEPTOR(-VEIN DONOR-SPECIFIC PREIMMUNIZATION AND SKIN ALLOGRAFTS() HYBRIDOMAS DERIVED FROM MICE GIVEN PORTAL)

Citation
Rm. Gorczynski et al., SPECIFICITY FOR IN-VIVO GRAFT PROLONGATION IN GAMMA-DELTA T-CELL RECEPTOR(-VEIN DONOR-SPECIFIC PREIMMUNIZATION AND SKIN ALLOGRAFTS() HYBRIDOMAS DERIVED FROM MICE GIVEN PORTAL), The Journal of immunology, 159(8), 1997, pp. 3698-3706
Citations number
46
Categorie Soggetti
Immunology
Journal title
The Journal of immunology
ISSN journal
00221767 → ACNP
Volume
159
Issue
8
Year of publication
1997
Pages
3698 - 3706
Database
ISI
SICI code
0022-1767(1997)159:8<3698:SFIGPI>2.0.ZU;2-3
Abstract
gamma delta TCR+ hybridoma cells prepared from mesenteric lymph node c ells of animals receiving donor-specific immunization via the portal v ein can adoptively transfer this increased graft survival to naive ani mals. Analysis of TCR gamma-chain junctional sequence diversity sugges ted that some 40 to 50% of the hybridomas expressed gamma-chain juncti onal sequence diversity and were stimulated to produce cytokines both by heat shock proteins and by minor histocompatibility Ag-specific irr adiated peritoneal cells. The remaining gamma delta TCR+ hybridoma cel ls expressed TCR with a common gamma-chain junctional sequence and wer e stimulated to cytokine production by MHC-matched, but minor histocom patibility Ag-mismatched (as well as matched), peritoneal cells, but n ot by heat shock proteins. We have compared the effectiveness of repre sentative hybridomas expressing unique gamma-chain junctional sequence s or common gamma-chain junctional sequences for prolongation of donor -specific or third-party (MHC-matched or MHC-mismatched) skin grafts. Our data show a good correlation between the specificity for stimulati on for cytokine production in vitro and efficacy in graft prolongation assays in vivo. Hybridoma cells expressing unique gamma-chain junctio nal sequences that showed Ag-specific stimulation of cytokine producti on in vitro and skin graft survival in vivo augmented survival of thir d-party skin grafts if simultaneously transplanted with both Ag-specif ic and third-party skin grafts. Graft prolongation in vivo using cells from either population of gamma delta TCR+ hybridomas was decreased b y infusion of anti-IL-10 mAb and abolished when both anti-IL-10 and an ti-TGF-beta Abs were used together.