CHARACTERIZATION OF THE HUMAN CD30 LIGAND GENE STRUCTURE

The gene for the human CD30 ligand was molecularly cloned, sequenced a nd characterised. The gene structure consisted of four exons and three intervening introns spaced over 17.1 kb of genomic DNA. The 5' flanki ng region of the CD30L gene was sequenced and analysis of the region r evealed the presence of several regulatory regions including a poly-dT element directly upstream from the transcription start site and conse nsus sequences for AP4, IK2, MZF1, E47 and ELK/cETS1. The absence of a canionical TATA motif suggests CD30L gene expression is regulated by a TATA-less promoter. (C) 1997 Elsevier Science B.V.