REFINED SUBCHROMOSOMAL LOCATION OF 21 EXPRESSED SEQUENCE TAGS FROM UNKNOWN GENES AT REGION 11P15

Twenty-one expressed sequence tags (ESTs) belonging to unidentified tr anscripts were mapped to 9 intervals at 11p15.5-p15.3. Thirteen were m apped to a region of an estimated size of 7 Mb with the help of a YAC contig. The remaining eight were mapped outside this region, and the c entromeric or telomeric position of the ESTs relative to the YAC conti g was defined with the help of a cellular hybrid containing a derivati ve chromosome 11 with a breakpoint within the region covered by the co ntig, The average size of the intervals, where the ESTs were mapped, w as estimated to be 0.3-0.4 Mb. The refinement of the chromosomal locat ion of these ESTs could be of great help in the identification of pote ntial candidate genes for disease locus mapping at 11p15.