CAPILLARY LC USING CONVENTIONAL HPLC INSTRUMENTATION

Capillary liquid chromatography (LC) using conventional high performan ce liquid chromatography (HPLC) instrumentation provides high reproduc ibility, high separation and detection sensitivities, and a large work ing range (conventional-, micro-, capillary-, and nanoscale) at modera te cost. Upgrading conventional HPLC instrumentation is easy and quick , and the resulting capillary LC systems can be used for protein-pepti de mapping at subpicomole levels. The low flow rates are compatible wi th high-sensitivity electrospray mass spectrometry (MS) detection and automated on-line micro-fraction collection onto matrix-assisted laser -desorption ionization targets with minimal risk of sample loss before MS analysis.