DIPHENYLTIN DICHLORIDE AS A CHROMOGENIC REAGENT FOR THE DETECTION OF FLAVONOIDS ON THIN-LAYER PLATES

Among several complexation reagents, diphenyltin dichloride (DTC) prov ed to be a useful chromogenic compound for both the qualitative and qu antitative analysis of flavones and flavonols on thin-layer plates by forming fluorescent complexes of different colour. For qualitative ana lysis, comparative detection with DTC and diphenylboric acid 2-aminoet hyl ester can indicate the position of the glycosidated hydroxyl of ka empferol and luteolin glycosides. Fluorimetric densitometry showed hig h sensitivity to flavonols with a free 5-hydroxyl group and flavones c ontaining two adjacent hydroxyl groups in ring B.